Digvijay Singh
K99 & Damon Runyon Postdoctoral Fellow
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I’m interested in molecular bits (the bases in a nucleic-acid sequence), molecular photography (capturing molecular structures and their locations in situ), & molecular videography (observing what molecules do, and how). My goal is to build platforms that advance molecular diagnostics, discovery, and therapy. I specialize in managing bits (the sequencing data and images), managing biomolecules (developing the assays), and managing photons & electrons (the light & electron microscopy that visualizes them). Lately, managing bits has increasingly meant orchestrating (& managing) neural networks with my biological insights. I think I am also good at managing people & relationships. In the age of artificial intelligence, I even more strongly commit myself to harnessing natural human-ness, so I place ever increasing emphasis on the management of people & relationships.


My academic geneology (incomplete)

Publications

Cryo-Focused Ion Beam Milling of Cells

The Molecular Architecture of the Nuclear Basket

Integrative spatiotemporal map of nucleocytoplasmic transport

Comprehensive structure and functional adaptations of the yeast nuclear pore complex

A viral genome packaging ring-ATPase is a flexibly coordinated pentamer

Effects of individual base-pairs on in vivo target search and destruction kinetics of bacterial small RNA

HSP70 chaperones RNA-free TDP-43 into anisotropic intranuclear liquid spherical shells

The SARS-CoV-2 nucleocapsid phosphoprotein forms mutually exclusive condensates with RNA and the membrane-associated M protein

Real-time observation of Cas9 postcatalytic domain motions

Preparing samples from whole cells using focused-ion-beam milling for cryo-electron tomography

Single molecule analysis of effects of non-canonical guide RNAs and specificity-enhancing mutations on Cas9-induced DNA unwinding

Real-time observation of DNA target interrogation and product release by the RNA-guided endonuclease CRISPR Cpf1 (Cas12a)

Mechanisms of improved specificity of engineered Cas9s revealed by single-molecule FRET analysis

Understanding the Molecular Mechanisms of the CRISPR Toolbox Using Single Molecule Approaches

The Single-Molecule Centroid Localization Algorithm Improves the Accuracy of Fluorescence Binding Assays

Real-time observation of DNA recognition and rejection by the RNA-guided endonuclease Cas9

Determination of in vivo target search kinetics of regulatory noncoding RNA

An Improved Surface Passivation Method for Single-Molecule Studies

Protein stability and folding kinetics in the nucleus and endoplasmic reticulum of eucaryotic cells