Digvijay Singh
K99 & Damon Runyon Postdoctoral Fellow
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I am interested in molecular photography (capturing molecular structures and their locations) and molecular videography (observing what molecules do and how). My goal is to develop platforms that advance molecular discoveries and therapies. I specialize in managing photons (light microscopy) and electrons (electron microscopy) and managing bits (i.e., the data from these microscopies, aka image processing).


My academic geneology (incomplete)

Publications

Cryo-Focused Ion Beam Milling of Cells

The Molecular Architecture of the Nuclear Basket

Integrative spatiotemporal map of nucleocytoplasmic transport

Comprehensive structure and functional adaptations of the yeast nuclear pore complex

A viral genome packaging ring-ATPase is a flexibly coordinated pentamer

Effects of individual base-pairs on in vivo target search and destruction kinetics of bacterial small RNA

HSP70 chaperones RNA-free TDP-43 into anisotropic intranuclear liquid spherical shells

The SARS-CoV-2 nucleocapsid phosphoprotein forms mutually exclusive condensates with RNA and the membrane-associated M protein

Real-time observation of Cas9 postcatalytic domain motions

Preparing samples from whole cells using focused-ion-beam milling for cryo-electron tomography

Single molecule analysis of effects of non-canonical guide RNAs and specificity-enhancing mutations on Cas9-induced DNA unwinding

Real-time observation of DNA target interrogation and product release by the RNA-guided endonuclease CRISPR Cpf1 (Cas12a)

Mechanisms of improved specificity of engineered Cas9s revealed by single-molecule FRET analysis

Understanding the Molecular Mechanisms of the CRISPR Toolbox Using Single Molecule Approaches

The Single-Molecule Centroid Localization Algorithm Improves the Accuracy of Fluorescence Binding Assays

Real-time observation of DNA recognition and rejection by the RNA-guided endonuclease Cas9

Determination of in vivo target search kinetics of regulatory noncoding RNA

An Improved Surface Passivation Method for Single-Molecule Studies

Protein stability and folding kinetics in the nucleus and endoplasmic reticulum of eucaryotic cells